Development of High-speed Optical Microscopes

By using advanced photon detectors such as HAPD and high-speed CMOS camera, Arisaka's group has been developing various types of high-speed optical microscopes in both wide Field of VIew and laser scanning confocal microscopes.

Spatio-Temporal Multiplexing (STEM) Microscope for Multiple Plane Imaging

Using HAPD with GaAsP photocathode, Arisaka and Adrian Cheng have developed an innovative two photon excitation microscopes called STEM shown below.

2PE

This allows to scan multiple planes simultaneously, utilizing a slight time difference (a few ns) between each beam which corresponds to each plane. The right side picture is the actual setup at Prof. Carlos Portera-Gailliau's Lab. The detail is given in the paper below:

Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,

Cheng A, Gonçalves JT, Golshani P, Arisaka K, Portera-Cailliau C., Nat Methods. 2011 Feb;8(2):139-42

High-speed CMOS Camera for Hair Cell Oscillation Measurements

At Prof. Dolores Bozovic's Lab, Arisaka has set up the fastest CMOS camera (SA-1) by Phorton , together with EMCCD Camera (Andor iXon 897). This system has been the work force in measuring the motion of multiple hair cells.

Doroles_CMOS

This system has resulted in several scientific papers, including the ones below:

Correlated movement of hair bundles coupled to the otolithic membrane in the bullfrog sacculus

Strimbu CE, Ramunno-Johnson D, Fredrickson L, Arisaka K, Bozovic D. Hear Res. 2009 Oct;256(1-2):58-63

Distribution of frequencies of spontaneous oscillations in hair cells of the bullfrog sacculus

Ramunno-Johnson D, Strimbu CE, Fredrickson L, Arisaka K, Bozovic D., Biophys J. 2009 Feb;96(3):1159-68

High-speed Confocal Microscope with Intensified CMOS at CNSI

At optical microscope core facility at CNSI, Arisaka has installed the ultra high-speed Image-Intencified CMOS camera, which was developed in collaboration with Photron, Japan. By combining with a Yokogawa Confocal Spinner as shown below, it is capable of taking 1,000 frame per second of confocal images.

CNSI_CMOS_Camera


Arisaka Lab's Activities in Bio-Imaging


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